Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: Primer sequences used for polymerase chain reaction.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: mRNA expression levels of (A) SHH, (B) Fgf8 and (C) Fgf10 in normal BALB/c mouse embryonic kidney tissue explants at E11.5, E12.5, E13.5 and E14.5 gestational ages. For each sample, the mRNA expression levels were normalized to GAPDH mRNA expression (n=7 for each treatment group ). * P<0.05, **P<0.01 and *** P<0.001 vs. E11.5 group. SHH, sonic hedgehog; Fgf, fibroblast growth factor; E, embryonic day.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: mRNA expression levels of (A) Fgf8 and (B) Fgf10 mRNA levels in BALB/c mouse kidney tissue explant cultures following treatment with 1% BSA, 1% BSA + SHH and 1% BSA + SHH + cyclopamine for 4 days. Fgf8 and Fgf10 expression levels were normalized to GAPDH mRNA expression levels (n=7 for each treatment group). ** P<0.01 vs. 1% BSA-alone group; ## P<0.01 vs. 1% BSA+SHH group. Fgf, fibroblast growth factor; BSA, bovine serum albumin; SHH, sonic hedgehog.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Sonic hedgehog protein regulates fibroblast growth factor 8 expression in metanephric explant culture from BALB/c mice: Possible mechanisms associated with renal morphogenesis

doi: 10.3892/mmr.2016.5614

Figure Lengend Snippet: Analysis of Fgf8 and Fgf10 expression in BALB/c mouse kidney tissue explant cultures following treatment with 1% BSA (control) or 1% BSA + SHH for 4 days. (A) Immunohistochemical analysis and (B) integral optical densities of Fgf8 and Fgf10 expression levels in the different treatment groups. (C) Western blot analysis and (D) quantification of western blot results, showing the protein expression levels of Fgf8 and Fgf10 expression levels in the various groups. The protein expression levels were normalized to β-actin (n=7 for each treatment group). Scale bar=125 µ m. * P<0.05 and ** P<0.01 vs. 1% BSA-alone group; # P<0.05 and ## P<0.01 vs. 1% BSA + SHH group. Fgf, fibroblast growth factor; BSA, bovine serum albumin; SHH, sonic hedgehog.

Article Snippet: Subsequently, sections were incubated overnight at 4°C with polyclonal rabbit anti-Fgf8 (dilution, 1:100; cat. no. ABIN1107218; Bioss Inc., Woburn, MA, USA) and polyclonal rabbit anti-Fgf10 (dilution, 1:100; cat. no. ABIN392510; Bioss Inc.) primary antibodies.

Techniques: Expressing, Immunohistochemical staining, Western Blot

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Western blots showing the specificity of the anti-FGF8 antibody. One hundred ng of FGF1 (lane 1), FGF2 (lane 2), FGF7 (lane 3), FGF9 (lane 4) and FGF8 (lane 5) were run on a 15% polyacrylamide gel and transferred onto nitrocellulose filters. These were probed with anti-FGF8 monoclonal antibody.

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Western Blot

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Expression of FGF8 in human breast cancer tissue. Paraffin sections of invasive ductal carcinoma ( A , B ), invasive lobular carcinoma ( C , D ) and ductal carcinoma in situ (DCIS) ( E , F ), were analysed by immunohistochemistry using an antibody against FGF-8 ( A , C , E ) or the equivalent concentration of non-immune mouse IgG ( B , F ). ( D ) Shows absence of anti-FGF8 staining after pre-incubation with 100-fold excess of recombinant FGF8. (Original magnification A – D , ×400; E , F , ×200).

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Expressing, In Situ, Immunohistochemistry, Concentration Assay, Staining, Incubation, Recombinant

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Expression of FGF8 in non-malignant human breast tissue. Paraffin sections of normal breast tissue from reduction mammoplasty tissue ( A , B ), fibroadenoma ( C , D ) and paraffin sections containing elements of both invasive ductal carcinoma and adjacent normal breast tissues ( E , F ), were analysed by immunohistochemistry using an antibody against FGF8 ( A , C , E , F ) or the equivalent concentration of non-immune mouse IgG ( B , D ). (Original magnification A – B , ×400; C – F ×200).

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Expressing, Immunohistochemistry, Concentration Assay

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Histograms showing the level of FGF8 immunoreactivity in malignant and non-malignant breast tissue. Tissues were scored as +, ++ or +++ for FGF8 staining. ( A ) Shows percentages of patients with each level of staining in: 1=Fibroadenoma; 2=Normal breast tissue (reducton mammoplasty); 3=Lactating breast tissue. ( B ) Shows FGF8 staining patterns in Invasive Ductal Carcinoma Grade 1, 2 and 3; DCIS and Lobular carcinoma.

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Staining

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: FGF8 staining in lactating mammary gland. Paraffin sections of lactating mammary gland tissue were stained with an antibody against FGF8 ( A , C ) or with the equivalent concentration of non-immune mouse IgG ( B , D ). (Original magnification A , B , ×200; C , D , ×400).

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Staining, Concentration Assay

Journal: British Journal of Cancer

Article Title: Fibroblast growth factor 8 is expressed at higher levels in lactating human breast and in breast cancer

doi: 10.1038/sj.bjc.6600213

Figure Lengend Snippet: Western blots demonstrating the presence of FGF8 in human breast milk. Proteins from human milk samples were run on a 12% polyacrylamide gel, transferred onto nitrocellulose and probed using the monoclonal antibody against FGF8. Lanes A – E contain milk proteins and lane F contains 100 ng of recombinant FGF8.

Article Snippet: The monoclonal anti-FGF8 antibody used in this study (R&D Systems) was produced from a mouse hybridoma in which the immunogen was an E. coli -derived recombinant mouse fibroblast growth factor 8b.

Techniques: Western Blot, Recombinant

Journal: Cells

Article Title: Expression of FGF8, FGF18, and FGFR4 in Gastroesophageal Adenocarcinomas

doi: 10.3390/cells8091092

Figure Lengend Snippet: Kaplan-Meier curves of overall survival of patients with adenocarcinomas of the esophago-gastric junction. ( a – c ) Patients from TCGA data set analysis: high FGF8, FGF18, and FGFR4 expression compared with those with low/absent FGF8, FGF18, and FGFR4 expression. ( d – f ) Patients from the immunohistological analysis: high FGF8, FGF18, and FGFR4 expression compared with those with low/absent FGF8, FGF18, and FGFR4 expression.

Article Snippet: Expression of FGF8, FGF18, and FGFR4 was detected by using polyclonal rabbit antibodies as follows: FGF8 antibody (Abcam, Cambridge, UK, ab203030) in a dilution of 1:600, FGF18 antibody (Assay Biotech, Fremont, CA, USA, C12364) in a dilution of 1:500, and FGFR4 antibody (Santa Cruz Biotechnology, Dallas, TX, USA, sc-124) in a dilution of 1:400, respectively.

Techniques: Expressing

Journal: Cells

Article Title: Expression of FGF8, FGF18, and FGFR4 in Gastroesophageal Adenocarcinomas

doi: 10.3390/cells8091092

Figure Lengend Snippet: Specimen of adenocarcinomas of the esophago-gastric junction stained for ( a ) FGF8, ( b ) FGF18 and ( c ) FGFR4. Positive staining was found in the tumor cells and to a lesser degree in the microenvironment. FGF8 and FGFR4 expression were primarily found in the nucleus, while FGF18 expression was mainly found in the cytoplasm. For quantitative evaluation, only epithelial cells were investigated. Corresponding sections stained by CK7 ( d ), Ki67 ( e ), and negative control ( f ). (The bar corresponds to 50 µm.) Original magnification ×400 all).

Article Snippet: Expression of FGF8, FGF18, and FGFR4 was detected by using polyclonal rabbit antibodies as follows: FGF8 antibody (Abcam, Cambridge, UK, ab203030) in a dilution of 1:600, FGF18 antibody (Assay Biotech, Fremont, CA, USA, C12364) in a dilution of 1:500, and FGFR4 antibody (Santa Cruz Biotechnology, Dallas, TX, USA, sc-124) in a dilution of 1:400, respectively.

Techniques: Staining, Expressing, Negative Control

Journal: Cells

Article Title: Expression of FGF8, FGF18, and FGFR4 in Gastroesophageal Adenocarcinomas

doi: 10.3390/cells8091092

Figure Lengend Snippet: Representative high ( a – c ) and low ( d – f ) expressing tumor section of FGF8 ( a and d ), FGF18 ( b and e ), and FGFR4 ( c and f ).

Article Snippet: Expression of FGF8, FGF18, and FGFR4 was detected by using polyclonal rabbit antibodies as follows: FGF8 antibody (Abcam, Cambridge, UK, ab203030) in a dilution of 1:600, FGF18 antibody (Assay Biotech, Fremont, CA, USA, C12364) in a dilution of 1:500, and FGFR4 antibody (Santa Cruz Biotechnology, Dallas, TX, USA, sc-124) in a dilution of 1:400, respectively.

Techniques: Expressing

Journal: Cells

Article Title: Expression of FGF8, FGF18, and FGFR4 in Gastroesophageal Adenocarcinomas

doi: 10.3390/cells8091092

Figure Lengend Snippet: FGF8, FGF18, and FGFR4 expression and their correlation with clinicopathologic parameters in patients with adenocarcinoma of the esophago-gastric junction.

Article Snippet: Expression of FGF8, FGF18, and FGFR4 was detected by using polyclonal rabbit antibodies as follows: FGF8 antibody (Abcam, Cambridge, UK, ab203030) in a dilution of 1:600, FGF18 antibody (Assay Biotech, Fremont, CA, USA, C12364) in a dilution of 1:500, and FGFR4 antibody (Santa Cruz Biotechnology, Dallas, TX, USA, sc-124) in a dilution of 1:400, respectively.

Techniques: Expressing, Adjuvant

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Evaluation and Immunolocalization of BMP4 and FGF8 in Odontogenic Cyst and Tumors

doi: 10.1155/2018/1204549

Figure Lengend Snippet: Evaluation and immunohistochemical score of stages of odontogenesis.

Article Snippet: The antibody clone used for detection of FGF8 was PA1216 (Boster, Pleasanton, CA, polyclonal antibody).

Techniques: Immunohistochemical staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Evaluation and Immunolocalization of BMP4 and FGF8 in Odontogenic Cyst and Tumors

doi: 10.1155/2018/1204549

Figure Lengend Snippet: Evaluation and immunohistochemical score of odontogenic tumors and odontogenic cyst.

Article Snippet: The antibody clone used for detection of FGF8 was PA1216 (Boster, Pleasanton, CA, polyclonal antibody).

Techniques: Immunohistochemical staining

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Evaluation and Immunolocalization of BMP4 and FGF8 in Odontogenic Cyst and Tumors

doi: 10.1155/2018/1204549

Figure Lengend Snippet: Intergroup comparison of expression of BMP4 and FGF8 in odontogenic tumors and odontogenic cyst.

Article Snippet: The antibody clone used for detection of FGF8 was PA1216 (Boster, Pleasanton, CA, polyclonal antibody).

Techniques: Comparison, Expressing